Fig. 5.

Immunogenicity of C2 and CM4 peptides by intramuscularly DNA immunisation

BALB/c mice were intramuscularly injected with 100µg L. mexicana gp63 cDNA twice. Two weeks after the last immunization they were killed and the splenocytes were cultured with APCs pulsed with C2 and CM4 peptides for 5 days. On day 5, the splenocytes were used as effectors in standard 4-hour cytotoxicity assay against tumor cells pulsed with the relevant peptides. Only 1 out of 6 mice showed immunogenicity against targets pulsed with C2 peptide. A: mouse immunized with C2 peptide B: mouse immunized with CM4 peptide. Graphs represent a number of independent positive experiments as Table 3