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B22R and B13R gene inactivation does not affect VV in vitro replication. Monolayers of BS-C-1 cells were infected at an MOI of 0.01, and at the indicated time points, intracellular (pelleted cells) (A) and extracellular (B) viral fractions (cell supernatants) were collected and their titers were determined. The data shown represent the mean values from triplicate samples assayed in duplicate; error bars indicate standard deviation.
