TABLE 1.
Oligonucleotide primers utilized in plasmid constructions in this study
| Name | Sequence (5′→3′)a |
|---|---|
| B13RF | TACACGACCAAGATCTATTACTATG |
| B13RR | TGAAAAGACGTGGGTACCAAAGTT |
| B22RF | GGTGGATCCCATGGATATCTTTAAA |
| B22RR | TATCAGATCTAGTTTATACTATTAC |
| pUC18F | TTCTTAGATCTCAGGTGGCACTT |
| pUC18/B13RR | AATACGGGTACCCACAGAATCAGG |
| pUC18/B22RR | ACAGAACCATGGGATAACGCAGG |
| p2B8RF | TAACAAACTAGTCCATAAGATCT CCC |
| p2B8R/B13RR | TTGTTTGTCGACTCTTATTATTTTTGACAC |
| p2B8R/B22RR | GTTTGCGGTACCTTATTATTTTTGACAC |
| gptF | ATAAACACCCGGGGACACTTCACAT |
| gptR | ACAACGCCCGGGAGTGCCAGG |
| gptS1 | AATATGAAAGTGGTGATTGTGA |
| gptS2 | ATGGGTTAAGACAATGACAAAT |
a
Engineered restriction endonuclease sites are underlined on the primer sequences.