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. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 28;48(6):1050–1059. doi: 10.1016/j.yjmcc.2010.01.001

Figure 5. Three alternatively spliced variants of αII-spectrin are expressed independently in rat heart.

Figure 5

Figure 5

A. Eight plasmid DNAs containing 3.7 kb of RT-PCR products, amplified by the primers listed in the table 1, were placed onto Nybond-N+ membrane and hybridized to the αII-spectrin probe, which was shared by all variants (A, top row) and showed positive in all clones. The membrane was then stripped and rehybridized with probes specific to the SH3+ (A, middle row) and Cardi+ (A, bottom row) variants. The probes used, and the scores for labeling of each plasmid, are summarized in the table below the blots. For information on the probes used, please see Supplementary Table 1. B. Additional sequencing of all RT-PCR products was performed. These experiments confirmed the results of Southern blots and also showed the presence or absence of the 5 amino acid insert in repeat 15 (insert 2) and the presence of the 6 amino acid insert (insert 3) in repeat 21 in all sequenced clones, which could not be assayed by blotting. The results indicate that the expression of mRNAs encoding the SH3+ insert (insert 1), the 5 amino acid insert in repeat 15 (insert 2), and the cardi+ insert in repeat 21 (cardiac-selective) are not linked. *The conventional isoform name is given in column 2, identifying αIIΣ9 and αIIΣ10 as new isoforms of αII-spectrin, according to previously identified isoforms [1].