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. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 28;48(6):1050–1059. doi: 10.1016/j.yjmcc.2010.01.001

Figure 6. Binding of αII-cardi+ to β-spectrins in blot overlay.

Figure 6

A. MBP-αII-cardi+ and MBP- αII-cardi− fusion proteins (2 μg each) were separated by SDS-PAGE, blotted with GST-βII-spectrin-1-4 (6.6 nM) and then with antibodies to GST (left) or to MBP (right, loading control). The results reveal that MBP-αII-cardi− binds more avidly than MBP-αII-cardi+ to GST-βII-spectrin-1-4 (6A. left). Similar amounts were present on the blot, as indicated by antibodies to MBP (6A right). B. Quantitations of blot overlays in A (n=4). The differences in binding of the αII-cardi− and αII-cardi+ fusion proteins are significant (*, p<0.05).