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. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Immunol. 2011 May 13;186(12):6683–6692. doi: 10.4049/jimmunol.1100525

Figure 1. Indirect presentation of the male HY alloantigen.

Figure 1

B6 and B6.129-IAb−/− female mice were immunised with either male donor 129/SvJ or 129-Ab0 splenocytes. After 7d, IFNγ response by TH cells to pHY/Ab or negative control pHX/Ab was assessed by ex vivo ELISpot assay. At the same time, IFNγ response by CTL to pH60/Kb, pH4b/Kb and negative control SV40 epi IV/Kb was similarly determined. Data represent 6 similar experiments using ~4 recipient mice per group per experiment; ± sem (standard error of mean).