Skip to main content
. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Immunol. 2011 May 13;186(12):6683–6692. doi: 10.4049/jimmunol.1100525

Figure 2. Indirect presentation of the class II-restricted HY alloantigen requires CD8+ DCs.

Figure 2

(a) Female recipient B6.FVB-hDTRtg mice treated with vehicle (PBS) or DT and immunised 24 hrs later with male donor 129.FVB-hDTRtg splenocytes from mice that were either PBS- or DT-treated 24 hrs earlier. After 7d, IFNγ response by TH cells to pHY/Ab or negative control pHX/Ab was assessed by ex vivo ELISpot assay. Data represent 6 similar experiments using ~3 recipient mice per group per experiment; ± sem. (b) Male donor 129.FVB-hDTRtg mice treated with vehicle or DT and used 24 hrs later to immunise female B6.FVB-hDTRtg mice. After 7d, IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELISpot assay. Data represent 6 similar experiments using ~3 recipient mice per group per experiment; ± sem. (c) 129/SvJ and 129-Batf30 female recipients were immunized with male C57BL/6 donor splenocytes. After 7d, IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELIspot assay. Data represents 2 similar experiments using ~2—4 recipient per group per experiment; ± sem. (d) B6 female recipients were immunized with male 129/SvJ or 129-Batf30 donor splenocytes. After 7d, IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELIspot assay. Data represents 2 similar experiments using ~2—4 recipient per group per experiment; ± sem. (e) 129/SvJ and 129-Batf30 female recipients were immunized with either male 129/SvJ or 129-Batf30 donor splenocytes. After 7d, IFN-γ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELIspot assay. Data represents 2 similar experiments using ~2—4 recipient per group per experiment; ± sem.