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. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Immunol. 2011 May 13;186(12):6683–6692. doi: 10.4049/jimmunol.1100525

Figure 3. ERAAP and TAP impact indirect presentation of class II-restricted HY alloantigen.

Figure 3

(a) B6 and B6.129-TAP0 female mice were immunised with either male donor C.B10-H2b or B.129-TAP0 splenocytes. IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELISpot assay after 7d. Data represent 8 similar experiments using ~3—4 recipient mice per group per experiment; ± sem. (b) B6, B6.129-TAP0 and B6.129-ERAAP0 female mice were immunised with either male donor C.B10-H2b or B.129-TAP0 splenocytes and IFNγ response by TH cells to pHY/Ab and pHX/Ab was assessed 7d later by ex vivo ELISpot assay. Data represent 7 similar experiments using ~4 recipient mice per group per experiment; ± sem. (c) B6, B6.129-TAP0 and B6.129-ERAAP0 female mice were immunised with either male donor 129S6/SvEvTac or 129-ERAAP0 splenocytes. IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined 7d later by ex vivo ELISpot assay. Data represent 7 similar experiments using ~2—3 recipient mice per group per mice; ± sem. (d) B6 and B6.129-ERAAP0 female mice were immunized with male donor 129S6/SvEvTac or 129-ERAAP0 splenocytes. After 7d, IFNγ response by TH cells to pHY/Ab and pHX/Ab was determined by ex vivo ELISpot assay. Data represent 3 similar experiments using ~2—3 recipient mice per group per experiment; ± sem.