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. Author manuscript; available in PMC: 2013 Jan 4.
Published in final edited form as: J Immunol. 2011 May 13;186(12):6683–6692. doi: 10.4049/jimmunol.1100525

Figure 6. Proteasomes regulate indirect presentation of the class II-restricted pHY alloantigen.

Figure 6

(a) B6 female mice were immunised with either DMSO (vehicle)- or epoxomicin-treated male donor 129/SvJ splenocytes. After 7d, IFNγ response by TH cells to pHY/Ab and pHX/Ab and by CTL to pH60/Kb, pH4b/Kb and SV40 epi IV/Kb was determined by ex vivo ELISpot assay. Data represent 5 similar experiments using ~2—3 recipient mice per group per experiment; ± sem. (b) 129/SvJ male donor splenocytes were treated for 2 hours with either DMSO (vehicle) or epoxomicin. B6 and B6.129-LMP20 female mice were immunised with extensively washed male donor 129/SvJ splenocytes. After 7d, IFNγ response TH cells to pHY/Ab and pHX/Ab was assessed by ex vivo ELISpot assay. Data represent 2 similar experiments using ~3 recipient mice per group per experiment; ± sem.