Figure 7. Donor HSP90, HSP70 and calreticulin facilitate indirect presentation of class II-restricted HY alloantigen.

(a) B6 female mice were immunised with Hsf1⁰Dby⁺, Hsf1⁺Dby⁺ or Hsf1⁰Dby⁻ MEF. After 7d, IFNγ response by Th cells to pHY/A^b and pHX/A^b was determined by ex vivo ELISpot assay. Data represent 2 similar experiments using ~2—3 recipient mice per group per experiment; ± sem. (b) Donor 129/SvJ splenocytes were treated with pharmacological inhibitors for 3 hours and next extensively washed with PBS. B6 female mice were immunised with either DMSO (vehicle), geldanamycin (HSP90 inhibitor) or KNK437 (HSP70 inhibitor) treated male donor 129/SvJ splenocytes. After 7d, IFNγ response Th cells to pHY/A^b and pHX/A^b was assessed by ex vivo ELISpot assay. Data represent 3 similar experiments using ~2—3 recipient mice per group per experiment; ± sem. (c) Female B6 mice were immunised with CRT⁺Dby⁺, CRT⁰Dby⁺, CRT⁺Dby⁻ or CRT⁰Dby⁻ MEF. After 7d, IFNγ response by T_H cells to pHY/A^b and pHX/A^b was determined as above. Data represent 3 similar experiments using ~3—4 recipient mice per group per experiment; ± sem.