Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 12;86(5):1167–1182. doi: 10.1111/mmi.12050

Figure 3. Effect of SD2 or SDA disruption on esp mRNA and protein levels.

Figure 3

(A) Mutations introduced into SD2 and/or SDA in plasmid pPL1.

(B – C) Northern blot analysis of (B) espAD and (C) espADB mRNA with oligonucleotide probes for espA (EspA4 or EspA3, respectively). In (C) RNA was extracted from EHEC strains at different OD600 values. Plasmid pmut-SD2-SDA has mutations in both SD2 and SDA. EHEC-mutSD2 has the SD2 mutations in the chromosome.

(D) Western blot analysis of EspA and EspB proteins. The immunoblot (left panel) was stained with Brilliant Blue R after EspA and EspB detection to show similar loading in each lane (right panel).