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. 2004 Mar;78(6):2906–2920. doi: 10.1128/JVI.78.6.2906-2920.2004

FIG. 5.

FIG. 5.

Reentry into the cell cycle results in loss of expression for class I IN mutants. (A) FIV vector. The percent LacZ expression over time is shown. Growth-arrested CrFK and HT1080 cells were treated with 15 μg of aphidicolin/ml 24 h before transduction. Arrested and dividing cells were transduced with RT-normalized stocks of IN mutant (D66V/D118A) and IN intact FIV lacZ vector at an MOI of 5. (B) HIV-1 vector. For comparison, arrested and dividing cells were also transduced with RT-normalized stocks of IN mutant (D64N/D116N) and IN intact HIV-1 lacZ vector at an MOI of 5. Percentages of cells expressing the lacZ transgene were measured for 3 weeks by fixing and staining them with X-Gal. Bars represent standard deviations of triplicate experiments.