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. 2012 Jun 22;12:118. doi: 10.1186/1471-2180-12-118

Figure 4.

Figure 4

Construction of M. smegmatis ΔgplH. (A) Scheme illustrating the deletion in the chromosome of Ms ΔgplH. The position of each primer used in this study is shown. (B) Scheme of the gplH deletion cassette-delivery suicide vector used for construction of Ms ΔgplH. The gplH deletion leaves behind a gene remnant coding for only the first 13 (black print) and last 4 (white print) amino acids of GplH. This gene remnant in ΔgplHc is flanked by ~1 kb of downstream and upstream WT sequence for homologous recombination with the chromosome. (C) Agarose gel electrophoresis showing PCR-based confirmation of the gplH deletion in Ms ΔgplH. Lanes: 1, Ms WT (2,239-bp amplicon expected with primers pepOF and pepOR); 2, Ms ΔgplH (2,068-bp amplicon expected with primers pepOF and pepOR); 3, Ms WT (278-bp amplicon expected with primers pepF and pepR); 4, Ms ΔgplH (101-bp amplicon expected with primers pepF and pepR); L, DNA ladder marker.