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. 2012 Nov 16;12:47. doi: 10.1186/1475-2867-12-47

Figure 1.

Figure 1

Effect of DOX and/or RSVL on apoptosis induction in MCF-7 cells. Apoptosis was analyzed after 48 h of exposure to drugs by staining with propidium iodide (PI, y-axis) and annexin- FITC (x-axis). (A) control, (B) cells treated with 15 μg/ml RSVL, (C) cells treated with 0.25 μg/ml DOX, (D) cells treated with 0.25 μg/ml DOX and RSVL 15 μg/ml in simultaneous manner, (E) cells treated with 0.25 μg/ml DOX and RSVL 15 μg/ml given 24 h before DOX. The percentage of cells in each quadrant are indicated (C1: necrosis, C2: late apoptosis, C3: live cells, C4: early apoptosis). The experiment was repeated twice each one in duplicate.