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. 2013 Jan 4;8(1):e53430. doi: 10.1371/journal.pone.0053430

Figure 2. JunB levels are increased in BSMC in response to TGFβ1, and in an ex vivo model of rodent bladder distension.

Figure 2

(A) BSMC were treated with TGFβ1 for the indicated times and assessed for JunB levels by immunoblotting. GAPDH is included as a loading control. (B) Immunofluorescence analysis of BSMC showing increased JunB nuclear localization upon TGFβ1 treatment for 24 h. (C) Sections from rat bladders distended ex vivo for 8 h (injured) were stained sequentially with anti-JunB and Cy3-conjugated species-specific secondary antibody. Increased nuclear fluorescent signal for both proteins was evident in the detrusor smooth muscle of stretch-injured specimens, but not of non-distended (control) bladders.