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. 2013 Jan 4;8(1):e53490. doi: 10.1371/journal.pone.0053490

Figure 5. Gene targeting.

Figure 5

(A) Schematic of the KO allele, in which exon 5 was deleted by homologous recombination in ES cells. (B) Behavior test for ataxia. Quantitative analysis of the duration the littermate wild-type mice (n = 8) and the Kif14 KO mice (n = 8) stood on a narrow platform. Error bars represent SD. (C) The Kif14 KO mouse phenotype. The littermate wild-type control mouse and the Kif14 KO mouse faced each other. Red arrow indicates the flat head of the Kif14 KO mouse. (D) Whole brain images of the littermate wild-type control mouse and the Kif14 KO mouse at P12. Arrowheads indicate the translucent olfactory bulb and spinal cord of the Kif14 KO brain. Bar, 5 mm. (E) Sagittal brain sections from the littermate wild-type control mouse (Ea) or Kif14 KO mouse (Eb) at P12 were immunostained with the anti-MBP antibody. Bars, 2 mm. (F) Sagittal brain sections from the littermate wild-type control mouse (Fa) or Kif14 KO mouse (Fb) at P12 were counterstained with hematoxylin. ac, anterior commissure; CX, cortex; DC, deep cerebellar nuclei; DG, dentate gyrus; fi, fimbria; Hi, hippocampus; Ic, inferior colliculus; OB, olfactory bulb; Pn, pontine nuclei; RMS, rostral migratory stream; Sc, superior colliculus; Spc, spinal cord. Bars, 2 mm.