FIG. 6.

Cholesterol depletion inhibits endosomal escape of Ad2 but does not affect endocytosis of ts1. wt Ad2 or ts1 Ad2 was bound to control HeLa cells, mβCD-treated HeLa cells, or mβCD-treated HeLa cells with cholesterol replaced (mβCD → 0.1mM Ch) in the cold, internalized for 70 min, and analyzed by TEM. Virus particles at the plasma membrane (PM), in endosomes (endos), and in the cytosol (Cyt) were quantitated as described in the legend to Fig. 4. The analysis of total endosomal virus (tot) included the determination of particles in small endosomes (s; <150-nm diameter), medium-size endosomes (m; ∼300-nm diameter), and large multivesicular endosomes (l;, >300-nm diameter). The error bars indicate the corresponding standard errors of the mean.