FIG. 7.

Nuclear targeting of Ad2-TR is inhibited in cholesterol-depleted cells. Ad2-TR was bound to control or mβCD-treated HeLa cells in the cold and internalized for different times as indicated. The cells were fixed, and the subcellular distribution of Ad2-TR was determined by quantitative microscopy. The results are expressed as fluorescence per unit area. Representative examples of cells fixed at 0 and 60 min p.i. are shown in the inset. The nuclei are stained with 4,6-diamidino-2-phenylindole (blue). Bar = 10 μm. The error bars indicate the corresponding standard errors of the mean.