Table 1. Expression of differentially significant kidney proteins between control A/J vs control 129P3/J mice.
| Spot n°. | Protein | aMw (kDa)/pI Expt. | bTheor. | cNumber of peptides/Score | dDifference(P value) | eUniprot ID | fBiological Process |
| 2 | Aconitate hydratase, mitochondrial | 91/4.71 | 82.5/7.4 | 6/99 | ↑129 (0.046) | Q99KI0 | Metabolism |
| 119/280 | ATP synthase subunit beta, mitochondrial | 33/5.155 | 51.8/5.0 | 16/1129 | ↑129(0.038) | P56480 | Metabolism |
| 151 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | 36.5/5.1 | 35.8/5.4 | 8/434 | ↑129(0.011) | Q9D051 | Metabolism |
| 175 | Hydroxyacid oxidase 2 | 38.5/7.94 | 38.7/7.6 | 11/529 | ↑129(0.012) | Q9NYQ2 | Metabolism |
| 217/221 | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | 42.5/8.055 | 43.6/7.69 | 15/715 | ↑129(0.001) | P45952 | Metabolism |
| 269 | Homogentisate 1,2-dioxygenase | 50/7.2 | 50/6.85 | 6/105 | ↑129(0.011) | O09173 | Metabolism |
| 384 | Sarcosine dehydrogenase, mitochondrial | 95/6.14 | - | 18/583 | ↑129(0.041) | Q99LB7 | Metabolism |
| 116 | Beta-lactamase-like protein 2 | 32.5/8.885 | 32.8/5.9 | 14/198 | ↑129(0.043) | Q99KR3 | Metabolism |
| 7 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | 38.5/5.675 | 37.4/5.9 | 9/122 | ↑129 (0.018) | P62137 | Processes/Information pathways |
| 317/321 | Catalase | 58/7.37 | 59.7/7.7 | 6/103 | ↑129(0.032) | P24270 | Processes |
| 396 | Aldehyde dehydrogenase family 1 member L1 | 100/5.445 | 98.7/5.6 | 24/517 | ↑129(0.013) | Q8R0Y6 | Processes |
| 285 | Alpha-aminoadipic semialdehyde dehydrogenase | 52/6.22 | 55.9/6.0 | 13/374 | ↑129(0.001) | Q9DBF1 | Processes |
| 324/583 | 60 kDa heat shock protein, mitochondrial | 59.5/4.875 | 58/5.35 | 22/992 | ↑129(0.022) | P63038 | Information Pathways |
| 413 | Coatomer subunit delta | 63/6.2 | 57.2/5.9 | 7/206 | ↑129(0.009) | Q5XJY5 | Transport |
| 3 | ATP synthase subunit delta, mitochondrial | 15.5/2.82 | 51.7/5.0 | 4/188 | ↓129(0.022) | P56480 | Metabolism |
| 171 | Alcohol dehydrogenase [NADP+] | 38/7.51 | 36.5/6.9 | 17/775 | ↓129(0.049) | Q9JII6 | Metabolism |
| 189 | Sorbitol dehydrogenase | 40.5/6.885 | 38.2/6.6 | 13/853 | ↓129(0.041) | Q64442 | Metabolism |
| 209 | Isovaleryl-CoA dehydrogenase, mitochondrial | 42.5/6.6 | 43/6.3 | 12/535 | ↓129(0.000) | Q9JHI5 | Metabolism |
| 233 | Ornithine aminotransferase, mitochondrial | 45/5.72 | 45.8/5.7 | 10/255 | ↓129(0.024) | P29758 | Metabolism |
| 237 | Creatine kinase U-type, mitochondrial | 45/8.05 | 43.2/7.3 | 9/133 | ↓129(0.033) | P30275 | Metabolism |
| 60 | Lactoylglutathione lyase | 27/4.48 | 20.7/5.25 | 37/365 | ↓129(0.001) | Q9CPU0 | Processes |
| 190 | Phosphotriesterase-related protein | 41/6.58 | 39.2/6.2 | 7/293 | ↓129(0.029) | Q60866 | Processes |
| 205 | 40S ribosomal protein SA | 42.5/3.96 | 32.7/4.8 | 7/825 | ↓129(0.044) | P14206 | Processes |
| 99 | Proteasome subunit beta type-7 | 30/5.885 | 25.3/5.8 | 7/85 | ↓129(0.041) | P70195 | Information pathways |
| 385/386/520 | Meprin A subunit alpha | 93/5.43 | 77.2/5.9 | 11/187 | ↓129(0.020) | P28825 | Information pathways |
| 355 | Serum albumin | 69.5/5.33 | 65.9/5.53 | 15/635 | ↓129(0.022) | P07724 | Transport |
a
Experimental molecular weight (kDa)/pI of protein spot in the gel (Mean of min. and max.) based on the coordinates of landmark proteins. bTheoretical molecular weight (kDa)/pI of theoretical protein. cNumber of peptides identified and score. dDifferences in expression in relation to 129P3/J mice (↓ down-modulation; ↑ up-modulation); Individual P value after ANOVA. eIdentification is based on protein ID from IPI (international protein index) protein database (http://www.uniprot.org/). fCategory of protein based on its primary biological function according to Rison (2000) [18].