Table 1.
The reported bacteria with nitrilase activity in previous literature
| Bacteria | Formation type | Molecular weight (kDa) | Optimum pH / temperature (°C) | Stability pH / temperature (°C) | Substrate specificity | Km (M) | Reference |
|---|---|---|---|---|---|---|---|
|
Pseudomonas sp. |
Inducible |
- |
- |
7.4-8.8 / - |
N-Methyl/ethyl-3-cyano-4- methoxy-2-pyridone |
1.9×10-5 |
[27] |
|
Nocardia sp. NCIB 11216 |
Inducible |
560 |
8 / - |
- |
Aromatic nitrile |
- |
[29] |
|
Brevibacterium strain R312 |
Constitutive |
- |
7 / 35 |
- / below 30 |
- |
2.5×10-2 |
[30] |
|
Pseudomonas sp. 13 |
Inducible |
1,000 |
9 / 55 |
7-11 / below 60 |
β-Cyano-L-alanine |
8.3×10-2 |
[31] |
|
Acinetobaeter sp. APN |
Inducible |
- |
- |
- |
α-Aminonitriles |
3.4×10-2 |
[32] |
|
R. rhodochrous J1 |
Inducible |
78 |
7.6 / 45 |
- / 20-50 |
Aliphatic, aromatic nitriles |
- |
[33,34] |
|
Comamonas testosteroni sp. |
Inducible |
- |
- |
- |
Aliphatic nitriles |
- |
[35] |
|
A. faecalis JM3 |
Inducible |
275 |
7.5 / 45 |
7-8 / 20-50 |
Arylacetonitriles |
3.3×10-4 |
[36] |
|
Acinetobacter sp. AK226 |
Inducible |
580 |
8 / 50 |
5.8-8 / below 60 |
Aliphatic, heterocyclic nitriles |
1.2×10-2 |
[37] |
|
A. faecalis ATCC 8750 |
Inducible |
460 |
7.5 / 40-45 |
6.5-8 / below 50 |
Arylacetonitriles |
5.8×10-3 |
[38] |
|
R. rhodochrous PA-34 |
Inducible |
45 |
7.5 / 35 |
- / below 35 |
Aromatic, aliphatic nitriles |
4.5×10-2 |
[39] |
|
R. rhodochrous K22 |
Inducible |
650 |
5.5 / 50 |
- / below 55 |
Aliphatic nitriles |
1.9×10-2 |
[40] |
|
Klebsiella ozaenae |
Constitutive |
37 |
9.2 / 35 |
- |
Bromoxynil |
3.1×10-4 |
[41] |
|
Bacillus pallidus Dac521 |
Inducible |
600 |
7.6 / 65 |
6-9 / below 65 |
Aromatic nitriles |
9.2×10-4 |
[42] |
|
R. rhodochrous NCIMB 11216 |
Inducible |
45.8 |
8 / 30 |
- |
Aromatic nitriles |
2.1×10-2 |
[43] |
|
P. fluorescens DSM 7155 |
Inducible |
130 |
9 / 55 |
- |
Arylacetonitriles |
8.7×10-5 |
[44] |
|
N. globerula NHB-2 |
Inducible |
- |
- |
- |
Aromatic, unsaturated aliphatic nitriles |
- |
[45] |
|
P. putida |
Inducible |
412 |
7 / 40 |
6.5-8 / below 50 |
Arylacetonitriles |
1.3×10-2 |
[46] |
|
Pyrococcus abyssi GE5 |
Inducible |
60 |
7.4 / 80 |
4.5–8.5 / 60-90 |
Aliphatic dinitriles |
9.5×10-3 |
[47] |
|
Acidovorax facilis 72W |
Inducible |
570 |
- / 65 |
5-10 / below 60 |
Aliphatic dinitriles |
5.6×10-2 |
[48] |
|
Rhodococcus sp. NDB1165 |
Inducible |
- |
8 / 45 |
- / below 50 |
Aromatic and unsaturated aliphatic nitriles |
0.1 |
[49] |
|
Bradyrhizobium japonicum USDA110 |
Inducible |
455 |
- |
- |
Mandelonitrile, phenylacetonitrile |
2.6×10-4 |
[50] |
|
P. aeruginosa 10145 |
Inducible |
- |
- |
- |
Aromatic nitriles |
- |
[51] |
|
Halomonas nitrilicus sp. nov. |
Inducible |
- |
- |
- |
Arylaliphatic nitriles |
- |
[52] |
| Bacillus subtilis ZJB-063 |
Constitutive |
- |
- |
- |
Arylacetonitriles |
- |
[53] |
|
Alcaligenes sp. ECU0401 |
Constitutive |
376 |
8 / 40 |
- / below 50 |
Aliphatic and aromatic nitriles |
2.2×10-2 |
[54] |
|
P. fluorescens Pf-5 |
- |
138 |
7 / 45 |
- / below 65 |
Dinitriles |
1.8×10-2 |
[55] |
|
Streptomyces sp. MTCC 7546 |
Inducible |
- |
- |
- |
Aliphatic nitriles |
- |
[56] |
|
Arthrobacter nitroguajacolicus ZJUTB06-99 |
Inducible |
- |
6.5 / 40 |
- / below 50 |
Aliphatic and aromatic nitriles |
1.2×10-2 |
[57] |
|
R. erythropolis ZJB-0910 |
Inducible |
- |
7.5 / 30 |
- / below 30 |
β-Hydroxy aliphatic nitrile |
1.0×10-2 |
[58] |
|
Geobacillus pallidus RAPc8 |
Inducible |
600 |
- |
- |
Aromatic nitriles |
- |
[59] |
|
A. faecalis ZJUTB10 |
Inducible |
- |
7.7–8.5 / 35 |
- / below 35 |
- |
- |
[60] |
| A. faecalis MTCC 10757 | Inducible | - | 8 / 35 | - | Aliphatic and aromatic nitriles | - | [61] |
Note: “-” indicates no data available.