TABLE 1.
Primers used for construction of mutant viruses
| Primer | Sequencea | Restriction enzyme | Nucleotide position | GenBank accession no. |
|---|---|---|---|---|
| EDELF | 5′-CACAGAATTCCGGGGGCGTCCTCTTCAGGG-3′ | EcoRI | 111-129 | M14336 |
| EDELR | 5′-CACAGGATCCGCGCGGACGGGAGATAAAACGCC-3′ | BamHI | 1177-1156 | M14336 |
| EDELF2 | 5′-CACAAGGATCCCCCGCTCCGGCTTCGACG-3′ | BamHI | 2854-2871 | M14336 |
| EDELR2 | 5′-CACAAAGCTTGCTTCGAGCCGTCCGCCG-3′ | HindIII | 559-542 | D10452 |
| gM FOR | 5′-CACAGAATTCCGTCCGCCGCCATGTGC-3′ | EcoRI | 3596-3580 | X97257 |
| gM REV | 5′-CACACTCGAGCCATGTGCGATCGAAACGAACG-3′ | XhoI | 2381-2402 | X97257 |
| KAN950 NEWFW | 5′-TCCGGATCCCGATTTATTCAACAAAGCCAGG-3′ | BamHI | 1800-1819 | X06402 |
| KAN950 NEVRV | 5′-TTCGAATTCGCCAGTGTTACAACCAATTAACC-3′ | EcoRI | 2769-2747 | X06402 |
a
Underlining indicates the restriction enzyme site.