Table 2. Primer sequences and amplicon characteristics of Triticum aestivum (wheat) and Malus domestica (apple) cross-allergens.
| Gene | GeneBank accension number | Sequence 5′–3′ | Product size (bp) | Ta (°C) |
| Tri a 12 | X89827.1 | CCTTGCACCAACTGGTCTTT | 162 | 60 |
| CCAGGAGTCATGGGTTCATC | ||||
| Tri a 14 | AJ784902.1 | GGCGTCAAGAACCTCCATAA | 177 | 60 |
| GCTGCAGTCGATGTTGAGAC | ||||
| Mal d 3.02 | AY572532.1 | TGGCCAGGTGAGCTCCAA | 247 | 60 |
| TGGTGGAGGTGCTGATCTTG | ||||
| Mal d 4.02 | AY792613.1 | GTGTTACTTGTCAAGAAGAGCACAA | 126 | 58 |
| GCTCAATGAGATAATCCGCA | ||||
| PLA 2.23 | EEF48118.1 | TGGGAAGTATTGTGGGCTTT | 259 | 52 |
| AGCAACTAAAGCAGCCTCCA |