FIG. 6.

Adoptive transfer of purified IgG from mice with high levels of anti-Ad5 immunity. Serum or purified IgG from naive donor mice or donor mice preimmunized with two injections of 10¹⁰ vp of rAd5-Empty were adoptively transferred to groups of recipient mice (four mice per group) prior to immunization of the recipient mice with 10⁸ vp of rAd5-Env. (A) Vaccine-elicited immune responses were assessed by D^d/P18 tetramer binding to CD8⁺ T lymphocytes. (B) Ad5-specific NAb titers of these mice were assessed before and after the adoptive transfer.