FIG. 7.

Adoptive transfer of purified CD8⁺ T lymphocytes from mice with high levels of anti-Ad5 immunity. Purified CD8⁺ T lymphocytes [CD8 (+)] or splenocytes depleted of CD8⁺ T lymphocytes [CD8 (−)] from donor mice preimmunized with two injections of 10¹⁰ vp of rAd5-Empty were adoptively transferred to groups of recipient mice (four mice per group) prior to immunization of the recipient mice with 10⁸ vp of rAd5-Env. A group of mice with active anti-Ad5 immunity was included as a control. Vaccine-elicited immune responses were assessed by D^d/P18 tetramer binding to CD8⁺ T lymphocytes (A), Env peptide pool and P18 epitope peptide-specific IFN-γ ELISPOT assays (B), and Env-specific ELISAs (C). (D) Ad5-specific NAb titers of these mice were assessed before and after the adoptive transfer. This study was repeated three times.