Fig. 3.

Quantification of myotube alignment in the reconfigurable co-culture device after 6 days in culture. “M” represents myoblasts; “F” represents fibroblasts in each figure. Contact mode is represented by adjacent boxes touching one another and gap is represented by a space between boxes. (A) Graphical representation of degrees from parallel versus culture type. Lower values indicate more aligned myotubes. The average degree from parallel of the finger of the comb in the myoblast-fibroblast contact co-culture was significantly less (*p< 0.05) compared to all other groups, indicating that the myoblasts were more aligned. (B) Histogram representation of myotube alignment. The data for degrees from parallel is grouped into 6 bins. Lower bin numbers represent more aligned myotubes with the comb finger. Graph shows a skewed distribution of myoblasts and fibroblasts in contact toward lower degree numbers