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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: Circ Res. 2012 Oct 9;112(1):113–127. doi: 10.1161/CIRCRESAHA.112.275586

Figure 4. KLF6 transactivates ALK1 promoter.

Figure 4

A. KLF6 interacts with ALK1 promoter in HUVECs and this interaction is increased after vascular injury. Left, chromatin immunoprecipitation (ChIP) of KLF6 over the two main KLF6-sites rich regions of the ALK1 5′-proximal promoter in HUVECs. Right, KLF6 binding was measured by densitometry of the individual bands and values of the (KLF6-IgG)/Input ratios were represented in both the control situation and 3 hr after the endothelial denudation B. Dose-response effect of KLF6 on the transcriptional activity of ALK1 promoter in HEK293T cells (left) and Drosophila Schneider S2 cells (right). C. Effect of KLF6 on the transcriptional activity of 5′deleted constructs of ALK1 promoter. Left, a scheme of the different ALK1 promoter constructs shows the distribution of the KLF6 consensus binding sites found along the ALK1 promoter (black boxes). Right, transient co-transfection of KLF6 expression vector with different 5′deleted constructs of ALK1 promoter in both HEK293T and Schneider S2 cells.