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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: Circ Res. 2012 Oct 9;112(1):113–127. doi: 10.1161/CIRCRESAHA.112.275586

Figure 7. Role of ALK5 signaling in the wounding-induced ALK1 expression.

Figure 7

A. ALK5 expression levels in HUVEC surface after wound healing measured by flow cytometry. B. ALK5 mRNA levels after wound healing measured by real time PCR. C. ALK1 expression in HUVEC surface after wound healing in absence or presence of the ALK5 kinase inhibitor SB431542, measured by flow cytometry. D. Histogram of ALK1 expression index from panel C. E. Wound healing assay of HUVECs treated or not with SB431542. Right, quantification of the endothelial migration expressed as number of migrating cells per field. F. Activity of Id1 promoter after treatment with SB431542 in HMEC-1 cells previously transfected with the PGL2 reporter containing the Id1 promoter (pId1);, empty PGL2.