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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Dec 9;20(1):127–133. doi: 10.1038/nsmb.2468

Figure 1.

Figure 1

Description of the in vivo RBP capture procedure and identified mRNA binding proteins. (a) A schematic of in vivo capture of RBPs to identify mRNA binding proteins including an SDS PAGE gel stained with SYPRO Ruby dye. (b–d) Proteins identified by mass spectrometry (MS). Numbers in parentheses in parts (b–d) indicate the number of proteins in each category. Some proteins are found in multiple categories. (b) The distribution of 120 identified poly(A) RNA binding proteins between known mRNA binding proteins, new RNA binding proteins and new mRNA binding proteins previously known to interact with other types of RNA. (c) The functional categories of previously known mRNA binding proteins identified by the RBP capture assay. (Total of 54 proteins) (d) The types of RNA, other than mRNA, bound by the newly identified proteins (total of 49 proteins). For proteins closely associated with RNA biology, but for which a specific RNA binding partner has not been identified, or which are predicted to bind RNA based on structural homology to RNA binding proteins, the category “undefined RNA” has been used.