Table 1.
Approximate Time Taken for Each 3D Reconstruction Case
| Variable | Mouse embryo | Human liver carcinoma | Human hepatitis C liver | Single rat glomerulus |
|---|---|---|---|---|
| Time taken by human | ||||
| Sectioning time (approximately 50 sections/hour) (minutes) | 130 | 100 | 120 | 160 |
| Staining time (minutes) | 80 | 80 | 160 | 40 |
| Segmentation (optional) (minutes) | 180 | 120 | 180 | 180 |
| Total human time (minutes) | 390 (6.5 hours) | 300 (5 hours) | 460 (7.7 hours) | 380 (6.3 hours) |
| Computational time⁎ | ||||
| Slide scanning (1 image/slide apart from the rat glomerulus: 18 images/slide; 8 slides in total) | 3330 | 350 | 500 | 1080 |
| Rigid and nonrigid registration | 720 | 540 | 660 | 960 |
| Total computer processing time (minutes) | 4050 (67.5 hours) | 890 (14.8 hours) | 1160 (19.3 hours) | 2040 (34 hours) |
⁎
Computational processing is unsupervised by humans.