Table 1.
Overview of the 37 mixtures made from pristine DNA and/or DNA extracted from buccal swabs. In the mixture ratio column, 1, 5 and 10 correspond to 30 pg, 150 pg, and 300 pg, respectively. “+” represents a sporadic contamination of 6 pg of DNA. For all, but one, mixture types two samples were prepared, by using DNA of other contributors or by switching the major and minor component(s). The percentage of alleles shared between all donors (homozygotes counted as two) is presented per mixture
| Relatedness in mixture | Number of contributors | Mixture ratio | Relatedness of mixture components | Percentage of shared alleles | |
|---|---|---|---|---|---|
| Mixture 1 (%) | Mixture 2 (%) | ||||
| Unrelated (U) | 2 | 1:1 | U:U | 41 | 38 |
| 2+ | 1:1+ | U:U:U | 56 | 53 | |
| 3 | 1:1:1 | U:U:U | 56 | 53 | |
| 4 | 1:1:1:1 | U:U:U:U | 63 | 62 | |
| 2 | 5:1 | U:U | 41 | 38 | |
| 2+ | 5:1+ | U:U:U | 56 | 53 | |
| 3 | 5:1:1 | U:U:U | 56 | 53 | |
| 4 | 5:1:1:1 | U:U:U:U | 63 | 62 | |
| 2 | 10:1 | U:U | 41 | 38 | |
| 2+ | 10:1+ | U:U:U | 56 | 53 | |
| 3 | 10:1:1 | U:U:U | 56 | 53 | |
| 4 | 10:1:1:1 | U:U:U:U | 63 | 62 | |
| Related (R, brothers) | 2 | 1:1 | R:R | 59 | 77 |
| 2 | 5:1 | R:R | 59 | 77 | |
| 2 | 10:1 | R:R | 59 | 77 | |
| 3 | 5:1:1 | U:R:R | 61 | – | |
| 3 | 5:1:1 | R:R:U | 68 | 59 | |
| 3 | 5:1:1 | R:R:R | 84 | 84 | |
| 4 | 5:1:1:1 | R:R:R:U | 77 | 77 | |
| Total number of mixtures | 37 | ||||