Table 2.

Example of allele calls in virtual pool profiles generated from amplifications one to four

	Allele call (rfu)
	10	11	15	16	18
Amplification 1	100	80	200	300	55
Amplification 2	nd	nd	150	150	nd
Amplification 3	150	nd	300	200	nd
Amplification 4	100	120	100	450	nd
Average peak height	88	50	188	275	14
Allele calls in virtual pool	10	11	15	16	–

nd allele not detected: not present or below the detection threshold of 50 rfu