Table 8.
Results for mock casework mixtures (contact traces with two donors). Amplification was carried out using 4 μl and 10 μl of each DNA extract, and CE analysis was performed using standard and higher CE injection settings. The average percentages of detected alleles and the average numbers of drop-in alleles are shown per individual profile, consensus profile (n = 4 x = 2) and pool profile (blend of four)
4-μl PCR input | 10-μl PCR input | |||||||
---|---|---|---|---|---|---|---|---|
Standard CE | Increased CE | Standard CE | Increased CE | |||||
Percentage of detected alleles (%) | Number of drop-in alleles/profile | Percentage of detected alleles (%) | Number of drop-in alleles/profile | Percentage of detected alleles (%) | Number of drop-in alleles/profile | Percentage of detected alleles (%) | Number of drop-in alleles/profile | |
Individual profiles (n = 16) | 65 ± 11 | 0.5 ± 1.0 | 81 ± 9 | 2.9 ± 2.2 | 86 ± 9 | 2.1 ± 2.0 | 93 ± 6 | 5.1 ± 2.5 |
Consensus profiles (n = 4) | 72 ± 13 | 0.0 ± 0.0 | 91 ± 6 | 1.8 ± 2.4 | 90 ± 8 | 1.8 ± 2.1 | 97 ± 4 | 5.0 ± 3.2 |
Pool profiles (n = 4) | 67 ± 13 | 0.0 ± 0.0 | 87 ± 8 | 1.5 ± 1.7 | 90 ± 7 | 0.5 ± 1.0 | 96 ± 5 | 3.0 ± 2.9 |