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TLR4 down-regulates antifibrotic miR-29. A: miRNA was isolated and subjected to qPCR analysis. Results, expressed relative to RNU24, are the mean ± SD of triplicate determinations. B: Normal skin fibroblasts were transfected with pre-miR-29b and incubated with LPS and TGF-β1 for 24 hours. Cell lysates were examined by using Western blot analysis. Representative immunoblots are shown. Cgn I, type I collagen.
