Reduced fibrotic gene expression in TLR4-mutant mice. C3H/HeJ and WT mice received daily s.c. injections of PBS or bleomycin (Bleo) for 21 days. Lesional skin was harvested and analyzed. A: RNA was quantitated by qPCR. The results, normalized for 36B4, represent the mean ± SD of triplicate determinations from four mice per group. Open bars, PBS; closed bars, Bleo. B: Collagen content determined by colorimetric assays. Results represent the mean ± SD of triplicate determinations from at least three mice per group. Open bars, PBS; closed bars, Bleo. C: Protein was examined by using Western blot analysis. Cgn I, type I collagen. *P < 0.05.