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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Hippocampus. 2012 Jul 20;23(1):40–52. doi: 10.1002/hipo.22052

Figure 6. Hyp2 induces membrane currents associated with Ca2+ elevations in hippocampal pyramidal neurons, as well as enhanced quantal transmitter release.

Figure 6

(A) Representative example of Hyp2-induced inward current and Ca2+ elevation in voltage-clamped fura-2-loaded CA3 pyramidal neurons (Vh: −65 mV; Cs-gluconate pipette; aCSF containing 500 nM TTX). Vehicle DMSO did not affect membrane conductance and Ca2+ levels.

(B) Average amplitude of Hyp2-induced inward currents (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Mann-Whitney U-test.

(C) Average increase in fura-2 ratio after Hyp2 application (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Mann-Whitney U-test.

(D) Hyp2-induced increase in mEPSC frequency (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Wilcoxon signed ranked test.

(E) Hyp2 did not affect mEPSC amplitude (n=8 cells) compared to DMSO controls (n=5 cells); p > 0.05 Wilcoxon signed ranked test.