(A) Representative example of Hyp2-induced inward current and Ca2+ elevation in voltage-clamped fura-2-loaded CA3 pyramidal neurons (Vh: −65 mV; Cs-gluconate pipette; aCSF containing 500 nM TTX). Vehicle DMSO did not affect membrane conductance and Ca2+ levels.
(B) Average amplitude of Hyp2-induced inward currents (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Mann-Whitney U-test.
(C) Average increase in fura-2 ratio after Hyp2 application (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Mann-Whitney U-test.
(D) Hyp2-induced increase in mEPSC frequency (n=8 cells) compared to DMSO controls (n=5 cells); p < 0.05 Wilcoxon signed ranked test.
(E) Hyp2 did not affect mEPSC amplitude (n=8 cells) compared to DMSO controls (n=5 cells); p > 0.05 Wilcoxon signed ranked test.