Figure 3.

Ex-vivo IL-17A production from γδ⁺T cells following neutrophil depletion. BALB/c mice received an intranasal inoculum of 1x10⁴ CFU of C. neoformans strain H99γ in 50 μl of sterile PBS. Mice were treated with isotype control antibody or with anti-1A8 antibody. The lungs were excised at day 7 post-inoculation and a single cell suspension generated using enzymatic digestion. The leukocytes were isolated and then further purified for γδ⁺ T cells. The γδ⁺ T cells were plated at 1 × 10⁵ cells/well and incubated for 24 h at 37°C, 5% CO₂ with media alone (white bars) or with C. neoformans cell wall extract (CWE) (gray bars). Cell supernatants were removed and examined for IL-17A by ELISA. Asterisks (*) indicate where significant differences (P < 0.05) were observed between γδ⁺ T cells from mice treated with isotype control antibody compared to those from mice treated with the 1A8 neutrophil depletion antibody. Data are cumulative of three separate experiments.