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Antibody reaction conditions and number of animals examined.
| Antibody | Dilution | Condition | Control | β1−/− | β2−/− | β3−/− | β4−/− |
|---|---|---|---|---|---|---|---|
| β1 | 1:100 | Overnight at 4° | 5 | 5 | n.a. | n.a. | n.a. |
| β2 | 1:50 | Overnight at rt | 5 | 4 | 5 | 2 | 5 |
| β3 | 1:500 | overnight at 4° | 5 | 4 | 2 | 5 | 4 |
| β4 | 1:100 | overnight at 4° | 5 | 4 | 4 | 4 | 5 |
rt (room temperature); n.a. not applicable.
