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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biol Cell. 2011 Oct 1;103(10):467–481. doi: 10.1042/BC20100146

Figure 2.

Figure 2

Networks formed by HMECs and HUVECs in culture are morphologically similar. Brightfield images (corresponding to 1h, 5h, 9h and 12h time points) taken from time-lapse movies of HUVECs (A) and HMECs (B) forming vascular networks on Matrigel. Sample fluorescent images of CellTracker Red-labeled HMEC cells at 5h (C) and 9h (E) are shown along with the corresponding object outlines (D, F, respectively), generated by MetaMorph. The largest “objects” (1 per image) are highlighted in white. (G, H) Quantification of network parameters using MetaMorph Integrated Morphometry Analysis module. For this analysis, number of objects and their average area were quantified, for each field of view (corresponding to the central region of the well), averaged for at least 16 wells and normalized to the 1h timepoints, separately for HMECs and HUVECs. Data are presented as mean +/− SEM.