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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biol Cell. 2011 Oct 1;103(10):467–481. doi: 10.1042/BC20100146

Figure 4.

Figure 4

Media effects on network formation by HMEC cells. (A) Representative pictures of networks formed by HMECs in multiple wells of a 96 well plate in various media for approximately 8–9 hours (except for the right-most PMA image which was taken at 20h post-plating). (B) Quantitation of the network formation using MetaMorph Integrated Morphometry Analysis. Discontinuinity of the networks is expressed as number of continuous objects normalized to EGM2-MV positive control condition and presented as average +/−SEM, minimum of 16 wells were analyzed per condition. Well formed, mature networks consist of small number of large objects, while disrupted networks and cell clusters are characterized by a larger number of disconnected smaller objects. (C, D) Quantitation of suramin effects on network formation. (C) Control cells (top panels) and cells treated with suramin (bottom panels), time points 0 and 8 hours are shown. (D) The parameters of cell structures formed after 8 hours were quantified using MetaMorph Integrated Morphology Analysis Module; comparisons of shape factor, length (μm) and outer radius (μm) for control and suramin are presented.