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. Author manuscript; available in PMC: 2013 Jan 6.
Published in final edited form as: Biol Cell. 2011 Oct 1;103(10):467–481. doi: 10.1042/BC20100146

Figure 5.

Figure 5

CellTracker labeled HMECs (top panel) exhibit higher photoxicity compared to the eGFP-expressing HMECs (lower panel). The images represent projections from z-stacks acquired with a 4x objective at the end of a 21 hour, 10-minute interval time lapse experiment set up using a 10x objective and a partially closed diaphragm. The illuminated areas in the center of each well are outlined by dotted lines and the exposure times are indicated. For the right-most images, a 4D acquisition (z-stack over time) was set up, resulting in 700 msec combined exposure of the central areas.