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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: J Am Chem Soc. 2012 Dec 6;134(50):20521–20532. doi: 10.1021/ja309790w

Figure 1.

Figure 1

(A) Structures of glycoside hydrolase-directed activity- based probes (GH-ABPs). (B) C. thermocellum secretome samples, containing cellulosome proteins, were incubated at 1 mg/mL with individual GH-ABPs (75 μM). Click chemistry was used to append the fluorophores rhodamine-azide and - alkyne, and proteins were separated by SDS-PAGE and imaged to reveal the fluorescent ABP-labeled proteins. Control gels and protein abundance stains are shown in the Supporting Information.