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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: J Am Chem Soc. 2012 Dec 6;134(50):20521–20532. doi: 10.1021/ja309790w

Table 1.

Spectral count (SC) and protein count data for labeling by each individual GH-ABP and for the global analysis of the C. thermocellum cellulosomal secretome.


Activity-Based Probe

Data Category GH2a GH2b GH2c GH2d GH3a GH3b GH4a GH4b Global
Total Protein Count 74 91 38 84 219 177 41 23 499
Total Peptide SC 783 1044 338 1061 4035 2872 345 200 42254
Carbohydrate Active Protein Count 36 41 24 39 60 52 17 12 84
Carbohydrate Active SCA 471 599 284 630 1344 1101 211 142 6997
Dockerin Containing Protein Count 27 27 20 25 33 30 9 9 41
Dockerin Containing SCB 303 358 188 374 667 534 100 71 1705
GH Family Protein Count 25 27 21 27 33 31 9 9 52
GH Family SCC 301 373 191 410 757 630 100 71 2233
A

Includes all enzyme families that act upon glycoside substrates, including glycoside hydrolases, glycosyl transferases, and carbohydrate kinases.

B

Includes all enzymes that contain a type I dockerin module for binding within the cellulosome macromolecular structure.

C

Includes all glycoside hydrolase enzymes, including those that are secreted but do not contain a type I dockerin module.