Fig. 4.

VBF and VIP1 levels affect the efficiency of uncoating of synthetic T-complexes. (A) Effect of E. coli challenge. (B) Effects of additional VBF and VIP1. Protein extracts from WT or VBF transgenic plants (VBF) untreated or challenged for 10 h with E. coli were incubated for the indicated time periods in the presence or absence of 1 μg of recombinant VIP1 purified as described previously (19) with synthetic T-complexes, and the susceptibility of ssDNA to DNase I was analyzed by PCR (Upper) and quantified by qPCR (Lower). The quantified data were expressed as percent of the input signal and represent average values of three independent experiments with indicated SDs. n/a, not applicable.