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Ca-ATPase activity of unphosphorylated and phosphorylated recombinant AMII constructs
| Construct | Ca-ATPase activity, s−1 |
|
| Unphosphorylated | Phosphorylated | |
| WT | 4.1 | 3.9 |
| ΔNHT | 3.1 | 3.4 |
| HMM | 3.4 | 3.9 |
| S1 | 3.5 | 3.1 |
| S639A | 3.4 | 4.1 |
| S639D | 3.8 | 3.6 |
| 4S/E | 3.6 | 3.4 |
Activity was determined in the absence of Mg2+ and F-actin as described in Materials and Methods.
