Fig. 4.

Broad-range conversion of fatty acids to fatty alcohols and alkanes. (A) In vitro formation of fatty alcohols. Reactions were carried out in 50 mM Tris⋅HCl buffer containing CAR_his (10–200 μg·mL⁻¹), Ahr_his (10 μg·mL⁻¹), 1 mM ATP, 10 mM MgCl₂, 1 mM NADPH, and 0.25 mM C₆–C₁₆ fatty acids. (B) In vitro rate of conversion of fatty acids to alcohols. Reactions were monitored at 340 nm for 6 min at 30-s intervals. (C) In vitro formation of alkanes. Same as A except Ahr_his was replaced with ADC_his. In A and C, the percentage yield of product per substrate over a 4-h period is indicated in brackets above each peak.