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. 2012 Dec 17;110(1):E33–E40. doi: 10.1073/pnas.1219727110

Fig. 10.

Fig. 10.

Isolated eWT AMII forms a heterogeneous mixture of minifilaments. Purified eWT monomers assembled in 10 mM imidazole (pH 7.0), 2.5 mM KCl, 1 mM ATP, and 1 mM MgCl2 form a mixture of clumped and staggered tetramers, hexamers, and octamers. Two images are shown for each structure; structures are identified in the left panels. (Scale bar, 100 nm.)