Table 1.
Effect of ionic conditions on the assembly of WT and pWT AMII
| Condition | Monomer (%) | Dimer (%) | Tetramer (%) | Hexamer (%) | Octamer (%) |
| 0.25 mM KCl | |||||
| WT | 11 | 10 | 41 | 39 | 0 |
| pWT | 90 | 9 | 1 | 0 | 0 |
| 0.25 mM KCl, 0.5 mM MgCl2 | |||||
| WT | 2 | 3 | 26 | 64 | 5 |
| pWT | 84 | 10 | 6 | 0 | 0 |
| 0.25 mM KCl, 1.0 mM MgCl2 | |||||
| WT | 0 | 0 | 3 | 13 | 84 |
| pWT | 3 | 1 | 79 | 5 | 12 |
| 0.25 mM KCl, 2.0 mM MgCl2 | |||||
| WT | 0 | 0 | 0 | 0 | 23* |
| pWT | 5 | 0 | 2 | 9 | 84 |
| 25 mM KCl | |||||
| WT | 0 | 0 | 20 | 30 | 50 |
| pWT | 0 | 0 | 80 | 15 | 5 |
All samples contained 10 mM imidazole (pH 7.0) and 1 mM ATP except for the 25 mM KCl sample. Percentages are based on more than 200 structures in 8–50 fields.
*The remaining filaments were larger than octamers.