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. 2012 Dec 10;110(1):E15–E22. doi: 10.1073/pnas.1214638110

Fig. 5.

Fig. 5.

Flow cytometry demonstrates specific binding of algal-produced immunotoxins. αCD22PE40 and αCD22CH23PE40 were incubated with CA-46 B cells, Ramos B cells, or Jurkat T cells. After primary incubation, cells were incubated with anti-exotoxin A produced in rabbit and finally with anti-rabbit DyLight 488. After incubation cells were analyzed by flow cytometry (blue curves). Cells that were not incubated with immunotoxins were used as a baseline of fluorescent intensity (red curves). (A) A shift in the fluorescence spectra demonstrates that αCD22PE40 and αCD22CH23PE40 bind to CA-46 B cells. (B) Fluorescence analysis also demonstrates that αCD22PE40 and αCD22CH23PE40 bind to Ramos B cells. (C) A lack of fluorescence shift demonstrates that algal-produced immunotoxins do not bind nonspecifically to Jurkat T cells.