Skip to main content
. 2012 Dec 17;110(1):306–311. doi: 10.1073/pnas.1210200110

Fig. 4.

Fig. 4.

GR knockdown increases eNOS expression. (A) Controls and GREC KO mice (n = 3 per group) were treated with vehicle or LPS 12.5 mg/kg and aortas were processed 8 h later. eNOS mRNA was significantly higher in GREC KO mice. (B) At baseline GR knockdown in HUVECs resulted in increased eNOS mRNA. After 24 h of treatment with LPS 10 μg/mL control cells showed a decrease in eNOS mRNA, as expected, whereas GR knockout cells continued to show increased mRNA levels. (C) Representative Western blot showing increased eNOS expression in GR knockdown cells both at baseline and after LPS treatment. (D) eNOS densitometry for three separate experiments. *P < 0.05 compared with nontreated control, **P < 0.05 compared with similarly treated control.