Table 2. Analysis of upstream DNA sequence elements in ActE genes upregulated during growth on cellulose.
| Locus | Catalytic domain | CBM | Annotated function | Sequencea | Rankb | Fold changeb |
|---|---|---|---|---|---|---|
| SACTE_0236 | GH48 | CBM2 | 1,4-beta cellobiohydrolase | TGGGAGCGCTCCCA | 1 | 21.7 |
| SACTE_0237 | GH6 | CBM2 | 1,4-beta cellobiohydrolase | TGGGAGCGCTCCCA | 2 | 17.3 |
| SACTE_3159 | CBM33 | CBM2 | Cellulose-binding domain | TGGGAGCGCTCCCA | 3 | 16.2 |
| SACTE_0482 | GH5 | CBM2 | Endo-1,4-beta-glucosidase | TGGGAGCGCTCCCA | 4 | 15.4 |
| SACTE_2288 | Transport systems inner membrane component | TGGGAGCGCTCCCA | 5 | 11.2 | ||
| SACTE_3717 | GH9 | CBM2 | 1,4-beta cellobiohydrolase | TGGGAGCGCTCCCA | 6 | 9.7 |
| SACTE_6428 | CBM33 | Chitin-binding, domain 3 | GGGAGCGCTCCCA | 9 | 7.9 | |
| SACTE_2347 | GH5 | CBM2 | Beta-mannosidase | TGGGAGCGCTCCCA | 11 | 5.0 |
| SACTE_2287 | Transport systems inner membrane component | TGGGAGCGCTCCCA | 15 | 4.3 | ||
| SACTE_2289 | Family 1 extracellular solute-binding protein | TGGGAGCGCTCCCA | 19 | 3.9 | ||
| SACTE_0352 | GCN5-related N-acetyltransferase | TGGGAGCGCTCCCA | 22 | 3.6 | ||
| SACTE_2286 | GH1 | Glycoside hydrolase 1 | GGGAGCGCTCCCA | 27 | 3.4 | |
| SACTE_0483 | CBM2 | Cellulose-binding family protein | GGGAGCGCTCCCA | 503 | 1.6 | |
| SACTE_0562 | GH74 | CBM2 | Secreted cellulase (endo) | TGGGAGCGCTCCCA | 5759 | 0.7 |
| SACTE_2285 | LacI family transcriptional regulator (CebR) | TGGGAGCGCTCCCA | 6229 | 0.6 | ||
aPredicted binding sequence element found upstream from gene locus.
bRanking and fold change in expression intensity detected by microarray for ActE genes when grown on cellulose relative to glucose.